CURRICULUM VITAE

Dr. SUKANYA V. SUBRAMANIAN

EDUCATION

Albert Einstein College of Medicine, New York, NY

1993 Ph.D. Cell and Molecular Biology

1990 MS Dept. of Anatomy and

Structural Biology

The All India Institute Of Medical Sciences, New Delhi, India

1985 MSc. Dept. of Anatomy

1983 BSc. Human Biology

RESEARCH AND PROFESSIONAL EXPERIENCE

Collin County Community College

2003-present Professor Biology Division of Math and Natural Sciences

(cell and molecular)

The Ohio State University, Columbus, OH

The Heart and Lung Institute,

2001 Research Scientist Dept. of Physiology and Cell biology

(Research Asst. Professor)

1997-2000 Postdoctoral Researcher Dept. of Cell Biology

Neurobiology and Anatomy

Studying the changes in protein and gene expression in cardiovascular development and disease as well as the gene transcriptional control mechanisms governing maladaptive cellular responses to injury and repair

Children's Hospital, Harvard Medical School, Boston, MA

1994 - 1996 Research Fellow Dept. of Medicine

Studied the regulation of a cell surface heparan sulfate proteoglycan, syndecan, in the process of wound healing by cell biological and biochemical approaches,

1993 - 1994 Research Fellow Dept. of Cardiology

Studied the role of MEF2 proteins in muscle development of mouse embryos to show the temporal and spatial expression of these proteins during development using whole mount immunocytochemistry

The Albert Einstein College of Medicine, New York, NY

1990 - 1993 Pre-Doctoral Student

Studied the signal transduction mechanisms of exocytosis in single celled eukaryotes using cell biological, biochemical and molecular biological techniques. Developed in vitro assays, for investigating post- translational modifications of signaling proteins.

The All India Institute of Medical Sciences, New Delhi, India

1985 - 1987 Pre-doctoral student Dept. of Reproductive Physiology

Studied the effect of drug treatments on the development of spermatozoa in animal model systems using biochemical and microscopic techniques.

1983- 1985 Masters student Dept. of Anatomy

Studied the development of corpus luteum in the primate using electron microscopic techniques

FELLOWSHIPS AND AWARDS

FELLOWSHIPS:

· NRSA (individual), 1998-99

· American Heart Fellowship, 1997-1998

· American Heart Fellowship, 1996-1997

· NRSA Grant (institutional) Post-Doctoral Trainee, 1994-1996

· NIH Grant (institutional) Pre-Doctoral Trainee, 1988-1993

· AIIMS Fellowship, 1985-1987

AWARDS:

· Sue Golding Award Lecture, 1992

· Travel Award, ASCB 1991

· Member, Physiology Course, MBL 198.

PUBLICATIONS AND PRESENTATIONS

PUBLICATIONS:

Subramanian, S.V., Polikandriotis, J. A, Kelm Jr.,R.J., David J.J. Orosz, C.G. and Strauch, A.R. 2004. Induction of Vascular Smooth Muscle {alpha}-Actin Gene Transcription in Transforming Growth Factor {beta}1-Activated Myofibroblasts Mediated by Dynamic Interplay between the Pur Repressor Proteins and Sp1/Smad Coactivators. Mol Biol Cell. 15(10):4532-4543

Subramanian, S.V., Kelm Jr., R.J., Polikandriotis, J. A, Orosz, C.G. and Strauch, A.R. 2002. Reprogramming of vascular smooth muscle a-actin gene expression as an early indicator of dysfunctional remodeling following heart transplant. *Cardiovascular Research 54: 539-548.

(*Editorial: Do we have an early marker of chronic transplant dysfunction now? W. Briest 2002. Cardiovascular Research, 54: 492-494)

Cogan, J. G., Subramanian, S.V., Polikandriotis, J. A, Kelm Jr., R.J. and Strauch, A.R. 2002. Vascular Smooth Muscle a-actin gene transcription during myofibroblast differentiation requires SP1/3 protein binding proximal to the cryptic MCAT enhancer. J Biol. Chem. 277: 36433-36442.

Strauch, A.R., Cogan, J.C., Kelm Jr., R.J., Subramanian, S.V. and Getz, M.J. 1999. Transgenic and Therapeutic targeting of smooth muscle cells and the challenges presented by phenotypic diversity. In Emerging Therapeutic Targets 3(2): 279-306.

Subramanian, S.V, Orosz, C.G. and Strauch, A.R. 1998. Vascular smooth muscle a-actin expression as an indicator of parenchymal cell reprogramming in chronic allograft rejection. Transplantation 65(12): 1652-1656

Strauch, A.R., Cogan, J.C., Subramanian, S.V., Armstrong, A.T., Sun, S., Kelm Jr., R.J. and Getz, M.J. 1997. Transcriptional activity of the vascular a-actin gene as an indicator of cellular injury following cardiac transplant. Transplant Immunology 5: 261-266

Subramanian, S.V., Fitzgerald M.L. and Bernfield, M. 1997. Regulated shedding of syndecan-1 and -4 ectodomains by thrombin and growth factor receptor activation.J Biol. Chem. 272: 14713-14720.

Subramanian, S.V. and Nadal-Ginard, B. 1996. The spatial and temporal regulation of MEF2 proteins in muscle and non-muscle cell lineages in developing mouse embryos. Mechanisms of Development 57:103-112

Subramanian, S.V., Wyroba, E., Andersen, A., and Satir, B.H., 1994. Cloning and sequencing of parafusin, a calcium-dependent and exocytosis related phosphoglycoprotein.

Proc. Nat'l Acad. Sci. USA 91:9832-9836.

Subramanian, S.V., and Satir, B.H., 1992. Carbohydrate cycling in signal transduction: Parafusin, a phosphoglycoprotein and possible calcium-dependent transducer molecule in exocytosis in Paramecium. Proc. Nat'l Acad. Sci. USA

Rajalakshmi, M., Sukanya, V., Ramakrishnan, P. R., and Kaur, J., 1990. Effect of Dihydrotestosterone on ultrastructural changes in rhesus monkey spermatozoa. Andrologia 22(2): 144-151

ABSTRACTS AND PRESENTATIONS:

 
Roy S, Khanna S, Bickerstaff A., Atalay M., Bieri M., Levy D., Subramanian S., Sharma N., Venojarvi M., Strauch A., Orosz C., and Sen C.K. Oxygen sensitive signalling in the heart. Wound Healing: Oxygen and Emerging Therapeutics, Columbus, OH, September 21-15, 2002

Subramanian,S.V., Polikandriotis, J. A., Cogan, J.G., Kelm Jr., R.J., Orosz, C.J. and Strauch, A.R. Myofibroblast activation contributes to transcriptional reprogramming of the VSM a-actin gene in chronically rejected cardiac allografts. Presented at Keystone symposium on inflammatory paradigms and vasculature II, Keystone, Colorado, 9-14 April, 2002

Cogan, J. G., Subramanian, S.V., Getz, M.J. and Strauch, A.R. 2000. The mouse

smooth muscle actin core promoter contains multiple Sp1/Sp3 binding sites required for basal and TGFb1-activated transcription in myofibroblasts. ASCB meeting, San Fransisco, CA, Dec. 2000.

Subramanian,S.V., Kelm Jr., R.J., Getz, M.J., Orosz, C.J. and Strauch, A.R 2000.

Chronic rejection is associated with altered expression of vascular smooth muscle actin transcriptional regulatory proteins. Transplant 2000 Joint meeting, Chicago, IL, May 13-17,2000

Subramanian,S.V., Kelm Jr., R.J., Getz, M.J., Orosz, C.J. and Strauch, A.R. 1999. The Pur-b ssDNA binding protein is implicated in the VSM alpha-actin gene reprogramming following Cardiac Transplant. Presented at keystone symposium, Keystone Colorado, 1999

Pelletier, R. P., Subramanian, S.V, Strauch, A.R. and Orosz, C. G. 1999. Conditions that influence parenchymal cell reprogramming associated with chronic allograft rejection! . Sixth Basic Sciences Symposium of The Transplantation Society Asilomar Conference Center, Monterey Peninsula, CA, August 25-29, 1999.

Pelletier, R. P., Subramanian, S.V, Strauch, A.R. and Orosz, C. G. 1999. Regulation of Parenchymal Cell Reprogramming associated with chronic allograft rejection. ASTP Annual Meeting, Chicago IL, May 15-19, 1999.

Strauch, A.R., Subramanian, S.V., Kelm Jr., R.J. and Getz, M.J. Transcriptional mediators of cardiac allograft remodeling. Proceedings of the Keystone Symposium on the molecular biology of the Cardiovascular system, Steamboat Springs, CO, 1 April 1998

Subramanian, S.V., Cogan, J. G., Orosz, C.G. and Strauch, A.R. 1998 Smooth muscle actin expression as an indicator of tissue remodeling after heart transplant. Proceedings of Experimental Biology ' 98 (Annual FASEB Meeting), San Fransisco, CA, 21 April 1998. FASEB Journal 12 (5,pt2): A716

Orosz, C.G., Subramanian, S.V. and Strauch, A.R. 1997.Non-traditional chronic rejection mechanisms. Proceedings of the international Congress on Immunosuppression, Orlando, FL, December 1997

Strauch, A. R., Armstrong, A, Subramanian, S.V and Orosz, C.1997. Cardiac allograft remodeling in the mouse in the Immunological Basis of Cardiovascular Disease. Keystone, Colorado, January 20-26, 1997.

Fitzgerald, M. L., Subramanian, S.V. and Bernfield, M. 1995. Shedding of syndecan extracellular domains from the cell surface occurs during injury and is regulated by agents involved in wound repair Mol cell Biol. 6 (suppl.)

Subramanian, S.V. and Nadal-Ginard, B. 1994. The spatial and temporal regulation of MEF2 proteins in developing mouse embryos. Mol. Cell Biol. 5 (suppl.), 2046a

Kaushal, S., Subramanian, S.V., Mahdavi V. and Nadal-Ginard, B.,1994.Myogenic function of MEF2 and cross regulatory network with the myogenic bHLH factors. Keystone Symposia on Molecular and Cellular Biology, Snowbird, Utah, April 11-17.

Subramanian, S.V., Wyroba, E., Andersen, A., and Satir, B.H., 1994. Cloning and sequencing of parafusin, a calcium-dependent phosphoglycoprotein involved in exocytosis. Mol. Cell Biol. 5, 25916a

Zhao, H., Subramanian, S.V., and Satir, B.H., 1993. In vitro regulation of membrane association/disassociation of parafusin via ATP phosphorylation. Mol. Cell Biol. 4 (suppl.):430a

Subramanian, S.V., and Satir, B.H., 1992. Enzymes involved in regulation of parafusin, a cytosolic phosphoprotein in Paramecium tetraurelia. Forum in developmental biology sponsored by the American Association off Anatomists, New York, March 11-15, 1992.

Subramanian, S.V., Andersen, A., and Satir, B.H., 1991. Localization of enzymes involved in glycosylation of parafusin, a cytosolic phosphoprotein involved in Paramecium exocytosis. J. Cell Biol. 115(3) part 2, 85a.

Subramanian, S.V., and Satir, B.H., 1991. Parafusin a 63 kD phosphoglycoprotein involved in Paramecium tetraurelia exocytosis undergoes a calcium dependent phosphorylation event in vitro. Fourth International Conference on Ciliate Molecular Biology, Asilomar Conference Center, Pacific Grove, CA, June 9-13, 1991.

Subramanian, S.V., and Satir, B.H., 1990. In vitro characterization of parafusin (63 kD phosphoglycoprotein) involved in Paramecium tetraurelia exocytosis. J. Cell Biol. 111(5) part 2, 76a.

Subramanian, S.V., and Satir, B.H., 1989. In vitro phosphorylation/dephosphorylation of parafusin in homogenates of Paramecium tetraurelia. First Annual Coast Regional Meeting of the Society of Protozoologists, Hofstra Univ., NY., June 2-4, 1989.